There are possibilities like structure based engineering of monoclonal antibody for improving solubility. Can we use these kind of engineering tools to improve specificity and sensitivity of a mAb, especially in case of rec mAb? Your valuable suggestions and comments are highly appreciated.
You can go for conserved epitope prediction and target that sequence to produce the antigen for the MAb production.
See
http://tools.immuneepitope.org/tools/conservancy.
To improve affinity and specificity by design, you would need a reliable structure of the complex. In my experience, you'll probably get there faster by in-vitro evolution. e.g. https://www.bioc.uzh.ch/plueckthun/index.php?pid=3-1-27700
@Rahul Krishnan
@Annemarie Honegger
Thanks for your valuable support and suggestions.
cheers,
Shyam
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