Surya Prasad Tiwari

3 Questions 12 Answers 0 Followers

Questions related from Surya Prasad Tiwari

What nutrient deficiency and/or (a)biotic stress and/or disease in Thale cress cause(s) the reddening of leaf stalk / petiole and/or silique stalk?

Hello everyone, I have grown Arabidopsis thaliana Col-0 and few other mutants. I have noticed different colours on the stalk of leaves & siliques as shown in the below pictures (A - Arabidopsis...

11 November 2017 5,841 5 View

Which (Coomassie or Silver staining) works best for SDS-PAGE gels ?

I have stained SDS-PAGE gels with Coomassie. But unfortunately, the bands were very faint / light blue and in some cases, it is very difficult to visualize at all after destaining after couple of...

01 January 1970 7,922 14 View

What could be the possible causes that my protein band size is very different from the expected protein size?

I perform western blot to separate proteins. The protein of interest is expected to be 27.66 kDa including the tags. However, I have got a clear band of size ~35 kDa. I’ve also repeated the...

01 January 1970 8,449 7 View