Shyam Kokkattunivarthil Uthaman

4 Questions 11 Answers 0 Followers

Questions related from Shyam Kokkattunivarthil Uthaman

Why the gold conjugated, specific monoclonal antibody is not reacting with the antigen in lateral flow immuno-chromatographic assay format?

The LFIA strip is not reacting to the corresponding antigen even at an addition of higher concentrations. The same mAb was used for AuNP conjugation and at the test line capture. The specificity...

12 December 2018 2,688 12 View

Why an unexpected single band in SDS-PAGE of purified IgG from ascitic fluid using AbFRONTIER Protein G Sepharose Fast Flow column?

I have tried to purify the IgG from mouse ascitic fluid using Abfrontier Protein G Sepharose fast flow column according to manufacture's protocol. The nanodrop reading is showing concentration of...

05 May 2018 8,202 8 View

Do we have any option to engineer the monoclonal antibody to increase its sensitivity and specificity?

There are possibilities like structure based engineering of monoclonal antibody for improving solubility. Can we use these kind of engineering tools to improve specificity and sensitivity of a...

04 April 2018 5,949 4 View

What is the difference between a primer used in the SYBR green based real time PCR and a TaqMan probe, if both are targeting a single gene?

Whether the same primer sequence (Used in the SYBRG-based qPCR) can be used to prepare TaqMan probe, if they are specifically amplifies the target? If not, what would be the exact difference or...

12 December 2017 2,995 6 View