What is the difference between 293t and 293tt cells ? furthermore, which one have superior transfection efficacy for the AAV vector ?
Nicolos explained the difference between 293t and 293tt cells very well. 293T cells is very suitable for AAV production. We always used 293T to detect the infctivity of packaged GFP-endoding AAV, most of AAV serotypes infect 293T very well except AAV2.
You could find more information about AAV production and application in this website: https://www.genemedi.net/i/aav-packaging
Hi Waqas,
293T cells carry a stably integrated copy of the SV40 genome and express an unspliced SV40 early mRNA that encodes small t antigen.
29TT cells carry a large T Antigen cDNA expression cassette. The expression cassette contains a hygromycin resistance gene. 293TT cells should be cultured in 250 µg/ml hygromycin to promote maintenance of Large T Antigen expression.
About AAV prodution I have no input but I expect that 293TT should be more efficient for plasmid replication and so, more efficient for AAV production if hygromycin selection does not interfer with the vector production process.
Regards
Nicolas
Nicolos explained the difference between 293t and 293tt cells very well. 293T cells is very suitable for AAV production. We always used 293T to detect the infctivity of packaged GFP-endoding AAV, most of AAV serotypes infect 293T very well except AAV2.
You could find more information about AAV production and application in this website: https://www.genemedi.net/i/aav-packaging
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