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Questions related from Waqas Nawaz
I used to perform the cytotoxicity assay. The short protocol is like that first I labeled the target cell with CFSE, Co-culture with CAR-t and then next day stained with PI and analyzed by FACS....
20 December 2018 2,905 3 View
I am generating Luciferase-expressing stable Tumor Cell Lines. First i co-transfected the Lentiviral plasmid containing the luciferase gene with 293t cell and produce the virus. Than i transduce...
19 December 2018 9,984 3 View
I conduct qPCR to determine the number of genome-containing particles in an AAV preparation using SYBR green technology. We used AAV-GFP plasmid as standard, and make 5 serial dilution (1:5) and...
21 November 2018 5,901 8 View
Hey All, I have a peptide composed of various domains. I have the sequence of whole peptide and individual domain. i wanna express that whole peptide on T cell. After expression, i wanna run the...
20 August 2018 826 17 View
I infected the 293tt cell with AAV virus to check the expression of my CAR molecule. According to protocols, I will collect the cells and wash in PBS, than i will stain with a antibody (Fab...
26 April 2018 4,439 5 View
I am going to transfect AAV293 cell with the AAV plasmid by adopting the PEI protocol. I wanna ask what is the best way to collect the viral stock ? In online literature and methods there are...
08 February 2018 4,716 3 View
What is the difference between 293t and 293tt cells ? furthermore, which one have superior transfection efficacy for the AAV vector ?
25 January 2018 1,667 6 View
