15 Questions 23 Answers 0 Followers
Questions related from Waqas Nawaz
Hey all, I am interested to study a transcription factor that usually present in cytoplasm; however; upon activation of certain signaling pathway its transfer to Nucleus. I want to study that...
06 October 2023 2,687 1 View
Hey all, I am using a transgenic mice. I want to run FACs to study the NK and NKT cell. What kind of markers will be best to detect the NK and NKT cells in blood from transgenic mice ? Thanks.
13 March 2023 6,440 1 View
I used to gate NK (CD56) and CD3+ cell in the same sample. According to previous literature, the NK cells are only 5-28% of the lymphocyte population in peripheral blood. However, I found that my...
04 July 2019 3,396 6 View
I am intended to perform Lymphocyte mediated serial cytotoxicity assay. I need to keep my PBMC for longer time, I am going to evaluate that for how many iterations my effectors cells (Lymphocyte)...
28 June 2019 179 1 View
I used to gate CD4 and CD8 from CD3+ cells. In my recent experiment i wanna gate CD4, CD8 and NK cells from PBMC. can someone recommend me the gating path for NK cell? Thanks.
04 January 2019 6,221 1 View
I used to perform the cytotoxicity assay. The short protocol is like that first I labeled the target cell with CFSE, Co-culture with CAR-t and then next day stained with PI and analyzed by FACS....
20 December 2018 2,953 3 View
I am generating Luciferase-expressing stable Tumor Cell Lines. First i co-transfected the Lentiviral plasmid containing the luciferase gene with 293t cell and produce the virus. Than i transduce...
19 December 2018 10,053 3 View
I conduct qPCR to determine the number of genome-containing particles in an AAV preparation using SYBR green technology. We used AAV-GFP plasmid as standard, and make 5 serial dilution (1:5) and...
21 November 2018 5,940 8 View
Hey All, I am using AAV293t cell line, which is adherent kind of cell. This cell line is a derivative of HEK293 cell line. I got new cells stock and after passaging them for 3 to 4 time, i see...
09 September 2018 5,890 5 View
Hey All, I have a peptide composed of various domains. I have the sequence of whole peptide and individual domain. i wanna express that whole peptide on T cell. After expression, i wanna run the...
20 August 2018 888 17 View
Dear All, In my experiment, I used huPBMC NCG mice and I express chimeric antigen receptor on CD8 cells, I want to know the percentage of naïve, effector, central memory and effector memory...
09 July 2018 107 5 View
I infected the 293tt cell with AAV virus to check the expression of my CAR molecule. According to protocols, I will collect the cells and wash in PBS, than i will stain with a antibody (Fab...
26 April 2018 4,486 5 View
I am going to transfect AAV293 cell with the AAV plasmid by adopting the PEI protocol. I wanna ask what is the best way to collect the viral stock ? In online literature and methods there are...
08 February 2018 4,763 3 View
From last week when i culture HEK293 and AAV293 cells from cyropreserve tubes, i faced the problem of cell floating when i observed it on next day. Although i add mycoplasma inhibitor and carry...
05 February 2018 7,680 4 View
What is the difference between 293t and 293tt cells ? furthermore, which one have superior transfection efficacy for the AAV vector ?
25 January 2018 1,710 6 View
