2,4 xylidine can be used as an inducer for enzyme Laccase from actinomycetes. But in literature why is it indicated that 2,5 xylidine is a preferred inducer as compared to 2,4 xylidine ?
2,5 is the usual one, although I think that 2.4 might be also effective. Difficult to predict
An enzyme inducer is a molecule that promotes synthesis of this enzyme according to a specific mechanism (e.g., Regulator ”protein” activation). In the case of laccase, 2,5-xylidine is more effective as inducer than the isomer 2,4-xylidine (experimental observation). This difference is probably due to the fact that the two methyl groups are more distant from each other in the isomer 2,5-xylidine than they are in the isomer 2,4-xylidine. Consequently, 2,5-xylidine is more specific in the laccase induction mechanism (link to the regulator and its activation).
I agree with Joseph. However, we should take into account several other circumstances. 1. Xylidine is inducer of laccase synthesis if it increases the respective gene expression.
2. It is possible that not 2,5- or 2,4-xylidine but the products of their transformation are inducers of enzyme synthesis, They are structurally different causing differential effect.
3. Both isomers are highly toxic and promotion of enzyme synthesis is a reaction of fungus to remove such toxic compound.
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