Are there any implications of choosing to use one of them on the statistical analysis later on?
Hey, I am wondering which tests I can do to analyze my results and if there is a better way to analyze my results. If I have multiple treatments in columns and I have just one donor in a row,...
30 April 2024 335 2 View
Hey, Usually, when I perform total RNA extraction, I directly synthesize cDNA and run qPCR without even analyzing or testing the purity of RNA. I am asked to use 15µl of RNA when synthesizing...
20 March 2024 1,380 2 View
I have a human primary keratinocyte cell line, and I want to incubate them with 4 and 24h with Cathepsin S. However, I have gone through literature but I didn't find papers talking about Cathepsin...
12 March 2024 2,051 3 View
Hey, I am currently doing my thesis and I am working with skin cells to detect a specific biomarker. I want to look for a biomarker in my collected media after treating them with 4 different...
11 January 2024 2,390 3 View
Most experiment can be done in chick embryo or growing chick and we can repeat the experiment and harvest brilliant results with low cost.
01 February 2018 2,809 3 View
Do you think two hours perfect time for transfer or you may lose the protein if you exceed?
01 February 2018 3,605 4 View
In one step q-PCR the reaction start with RNA while in two steps starts with cDNA
01 February 2018 4,767 6 View
Is it possible
01 February 2018 6,778 0 View
I am trying to investigate the effect of the tested compound/s on the activity of secreted interleukines in leukaemia cells, I did the experiment in two ways. The 1st was without any activation,...
31 December 2016 2,854 6 View
Please, can any one help me to learn primer design ? and what the best methods to do that or best program? Regards Israa
07 August 2016 7,519 10 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View