Hi, are you with or against performing experiments on Avain species as a laboratory animal ?

Most experiment can be done in chick embryo or growing chick and we can repeat the experiment and harvest brilliant results with low cost.

01 February 2018 2,660 3 View

Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

01 February 2018 4,547 6 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

01 February 2018 3,458 4 View

Can we use human antibodies for western blot of chicken cells?

Is it possible

01 February 2018 6,622 0 View

Do I have to stimulate/induce inflammation in cancer cells in order to measure Interleukins expression/regulation upon specific treatment?

31 December 2016 2,725 6 View

Please, can any one help me to learn primer design ?

Please, can any one help me to learn primer design ? and what the best methods to do that or best program? Regards Israa

07 August 2016 7,389 10 View

How can i caculate sample size using results from multiple linear regression that included an interaction term of interest??

I ran a multiple linear regression including x1 =(Continuous variable), x2 =(dummy variable where 0= male, 1= female, and x1*x2= (an interaction term of both) in addition to 2 other variables that...

01 February 2016 8,465 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View