I have a human primary keratinocyte cell line, and I want to incubate them with 4 and 24h with Cathepsin S. However, I have gone through literature but I didn't find papers talking about Cathepsin S concentrations in the context of human primary keratinocytes.
I am wondering about the concentration that I can test to see if I can induce a specific gene. Also, if there are useful papers please add the website for it.
Thank you