Hi, are you with or against performing experiments on Avain species as a laboratory animal ?

Most experiment can be done in chick embryo or growing chick and we can repeat the experiment and harvest brilliant results with low cost.

01 February 2018 2,660 3 View

Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

01 February 2018 4,547 6 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

01 February 2018 3,458 4 View

What is the main difference between Necrosis and Necroptosis in terms of epigenitics ?

Necrosis and Necroptosis are not the same from my understanding of molecular biology.

31 January 2018 2,522 5 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

31 January 2018 5,886 3 View

Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

31 January 2018 7,018 5 View

One of my papers has been removed from google scholar. what is the reason?

One of my papers is disappeared from google scholar last Wednesday. While its still published at IEEE xplore.

10 November 2017 7,236 5 View

Which method is good (in terms of accuracy) to solve a box-constrained optimization problem with 28 variables?

I have a 7 sensors uniform linear array that have some errors to be estimated (i.e. to do array calibration). The errors are: gain, phase, and position (in y and z axes). So, the total is 28...

06 July 2017 6,839 3 View

Do I have to stimulate/induce inflammation in cancer cells in order to measure Interleukins expression/regulation upon specific treatment?

31 December 2016 2,725 6 View

What is the minimum thickness for rubber tube? Could you write its specifications?

11 December 2016 4,719 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View