Hi, are you with or against performing experiments on Avain species as a laboratory animal ?

Most experiment can be done in chick embryo or growing chick and we can repeat the experiment and harvest brilliant results with low cost.

01 February 2018 2,660 3 View

Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

01 February 2018 4,547 6 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

01 February 2018 3,458 4 View

Can we use human antibodies for western blot of chicken cells?

Is it possible

01 February 2018 6,622 0 View

What is the main difference between Necrosis and Necroptosis in terms of epigenitics ?

Necrosis and Necroptosis are not the same from my understanding of molecular biology.

31 January 2018 2,522 5 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

31 January 2018 5,886 3 View

Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

31 January 2018 7,018 5 View

One of my papers has been removed from google scholar. what is the reason?

One of my papers is disappeared from google scholar last Wednesday. While its still published at IEEE xplore.

10 November 2017 7,236 5 View

Which method is good (in terms of accuracy) to solve a box-constrained optimization problem with 28 variables?

I have a 7 sensors uniform linear array that have some errors to be estimated (i.e. to do array calibration). The errors are: gain, phase, and position (in y and z axes). So, the total is 28...

06 July 2017 6,839 3 View

What is the minimum thickness for rubber tube? Could you write its specifications?

11 December 2016 4,719 2 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How can I add a new WSN routing protocol in NS2 or NS3?

I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...

19 February 2021 7,981 3 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View