Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

01 February 2018 4,547 6 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

01 February 2018 3,458 4 View

Can we use human antibodies for western blot of chicken cells?

Is it possible

01 February 2018 6,622 0 View

What is the main difference between Necrosis and Necroptosis in terms of epigenitics ?

Necrosis and Necroptosis are not the same from my understanding of molecular biology.

31 January 2018 2,522 5 View

Western blot transfer time?

Do you think two hours perfect time for transfer or you may lose the protein if you exceed?

31 January 2018 5,886 3 View

Which procedure much precise; one step or two steps q-PCR?

In one step q-PCR the reaction start with RNA while in two steps starts with cDNA

31 January 2018 7,018 5 View

One of my papers has been removed from google scholar. what is the reason?

One of my papers is disappeared from google scholar last Wednesday. While its still published at IEEE xplore.

10 November 2017 7,236 5 View

Which method is good (in terms of accuracy) to solve a box-constrained optimization problem with 28 variables?

I have a 7 sensors uniform linear array that have some errors to be estimated (i.e. to do array calibration). The errors are: gain, phase, and position (in y and z axes). So, the total is 28...

06 July 2017 6,839 3 View

Do I have to stimulate/induce inflammation in cancer cells in order to measure Interleukins expression/regulation upon specific treatment?

31 December 2016 2,725 6 View

What is the minimum thickness for rubber tube? Could you write its specifications?

11 December 2016 4,719 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View