Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

How to know whether SVM algorithms performed well?

When doing machine learning, do we normally use several algorithms for comparison? For example, if the RMSE of SVM is 0.1, how do I come up with the conclusion that this model performed well? Just...

29 February 2020 9,837 8 View

What is the typical way to analyze fraction value of RGB?

Hi experts! I am using Cloud Vision API and I have got the image properties for 4000+ pictures. Each of the picture consisted of 10 RGB value and fraction value. I wonder whtat is the most common...

25 October 2019 5,338 1 View

How many knockdown should you start with for proteomics?

Hello, I am working on a project with knockdowns. Is there a universal amount of knockdown one start with? Is there anything in literature to support this? I'm having trouble finding...

05 September 2019 1,430 1 View

How to map emotions on the circumplex model using biometrics?

I wonder is there any way to map emotions on the two-dimensional circumplex space mode based on valence and arousal generated from either heart rate or GSR? (or any other biometrics) I presume...

09 July 2019 4,255 8 View

A statistical test to compare frequencies, including zero values?

I want to quantify the difference between the frequency of contamination with four different microbes from two independent sources of data. One data source reported no contamination with any of...

24 April 2018 6,861 5 View

Anyone good with P2 mouse pup head anatomy - coronal section?

Hi all, I'm totally new to the wonders of mouse anatomy and histology. Can anyone direct me to a resource that can help me identify the structures (not the brain) in this pic (attached) - mouse...

07 March 2018 843 3 View

What is my design methodology?

I have become totally confused about my methodology section now I am writing it up. I know it's BAD! But the more I have read the more unclear it has become to me. I thought it was qualitative...

05 November 2017 6,963 5 View

Can a qualitative research study just use indepth interviews for data?

I have conducted 12 in depth interviews to analyse for my qualitative research study - does a qualitative study have to have more than one source of data?

03 October 2017 343 9 View

During extinction events (geologic time scale divisions), how do nutrients get moved around in ecosystems?

To think about this, consider that thousands of wildebeests drown annually in Africa, and this has a major effect on the movement of Nitrogen, Phosphorus, and Carbon in...

23 June 2017 9,734 8 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View