Do you know what happen in reaction between Ni2SO4.6H2O and DMG (dimethylgolyoxin)?

I want to use DMG as a reagent for detecting Ni in a solution. in their reaction they form a complex together which color is pink. I attached a picture of the reaction. my problem is I can`t find...

01 January 2021 7,200 3 View

How can i make a 1000 ppm Ni(II) solution?

I try to prepare 100ml of a 1000 ppm Ni(II) solution by solving NiSO4.6H2O in ionized water. To do so I add 0.45g of NiSo4.6H2O to the water. i would appreciate if anyone tell me whether it is...

31 August 2020 7,807 4 View

What are the antenna design challenges for 5G handset uses,these days?

any suggestion on recently designed antennas for 5G handset uses.

08 November 2019 5,509 4 View

How can i amplify fragment of approximately 3100 bp?

Hi, I want to amplify fragment of approximately 3100 bp from bacterial genome for cloning in pET28a using NcoI and XhoI restriction enzymes. I performed PCR using following program:...

24 July 2019 6,115 7 View

Is there any procedure to synthesis [(NH3)5 Ru SC6H5]2+ from RuCl3 and Diphenyl disulfide?

I need to prepare [(NH3)5 Ru SC6H5]2+ from RuCl3 and Diphenyl disulfide. So at first Ru(III) should be reduced to Ru(II) by zinc powder and then Ru(II) oxidize to Ru(III) by diphenyl disulfide to...

19 May 2019 1,064 1 View

Gold nanoparticle deposited on carbon printed electrode generate redox peak in the CV?

When i test the gold nanoparticle coated carbon printed electrode in the PBS, I see the redox peaks but the potential for the Au to reduce or oxidise is not in the range of my CV(-0.3 to 0.7). Can...

27 March 2019 7,519 3 View

What are the best compounds to increase neuronal proliferation?

I am working with Parkinson cell lines ( SH-SY5Y ) and stem cells, I have to find compounds that increase proliferation of neurons. Thank you

29 January 2019 539 3 View

Can someone explain what analysis has been used in this article ?

Hello I would appreciate it if someone explain the analysis which is used in order to find the association between two components with the control of another factor in this article . cause at...

16 November 2018 7,048 2 View

How many methods exist for conversion of Myoglobin to Apomyoglobin?

We need a method with the least waste of myoglobin.

02 October 2016 5,367 1 View

Why we use the idea of passivity-based controllers for nonlinear bilateral teleoperation systems?

A bilateral teleoperation system is composed of five interconnected elements, including a human operator, local and remote manipulators, communication channel and an environment. Human operator...

17 September 2016 7,758 10 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View