How to implement cytotoxicity test in microtiter plate?

I used 96 well and brine shrimp for cytotoxicity analysis of plant extract.After 48 hours hatching, the experiment was conducted. But the nauplli didn't survive even in the negative control....

31 December 2018 5,228 5 View

Is there any protocol to investigate Brine shrimp lethality test using microtiter plate?

I want to proceed cytotoxicity effect of plant extract which i found interesting against biofilm producing bacteria. Unfortunately, I've not much extract remain to implement test tube based...

11 December 2018 3,266 3 View

What is standard procedure to prepare plant extract stock solution for antimicrobial assay?

I'm following an article where they used 0.2% DMSO. I applied that but find out some problem as the dried extracts become sticky and don't dissolve at 100 mg/mL concentration. In another article,...

06 July 2018 1,707 4 View

What are the plant extracts that show both antibacterial and antibiofilm activity?

I need some papers( both research + review) where plant extracts been used both for both antibacterial and antibifilm candidate. Secondly, any database of secondary metabolites having...

03 April 2018 1,921 4 View

What's the protocol of TA cloning?

I'm looking for specific procedure of TA cloning. Best if someone provides this with published article.

01 February 2018 7,293 3 View

What's the standard procedure to prepare Agrobacterium stock solution?

In my lab, we lost the identification sign of Agrobacterium stock. So i tried to prepare stock by the following two procedures: 1. First i cultured Agrobacterium on solid YEP media and then...

10 November 2017 6,224 7 View

What are the nano-particles use for non-viral gene therapy?

08 September 2017 5,361 4 View

What's the reason i'm failing to install Autodock in windows 7?

04 May 2017 7,806 1 View

Is low validated protein model a problem for publication?

31 December 2016 4,394 6 View

Is it possible to conduct Agrobacterium mediated nitrogen fixing gene(nif gene) transfer into plant species?

Some bacteria naturally can utilize atmospheric nitrogen. My question is their any research is going to transfer nif gene into non-leguminous plant. Thanks in advance

07 August 2016 5,452 1 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View