How to implement cytotoxicity test in microtiter plate?

I used 96 well and brine shrimp for cytotoxicity analysis of plant extract.After 48 hours hatching, the experiment was conducted. But the nauplli didn't survive even in the negative control....

31 December 2018 5,228 5 View

Is there any protocol to investigate Brine shrimp lethality test using microtiter plate?

I want to proceed cytotoxicity effect of plant extract which i found interesting against biofilm producing bacteria. Unfortunately, I've not much extract remain to implement test tube based...

11 December 2018 3,266 3 View

What is standard procedure to prepare plant extract stock solution for antimicrobial assay?

I'm following an article where they used 0.2% DMSO. I applied that but find out some problem as the dried extracts become sticky and don't dissolve at 100 mg/mL concentration. In another article,...

06 July 2018 1,707 4 View

What's the protocol of TA cloning?

I'm looking for specific procedure of TA cloning. Best if someone provides this with published article.

01 February 2018 7,293 3 View

What's the standard procedure to prepare Agrobacterium stock solution?

In my lab, we lost the identification sign of Agrobacterium stock. So i tried to prepare stock by the following two procedures: 1. First i cultured Agrobacterium on solid YEP media and then...

10 November 2017 6,224 7 View

What are the nano-particles use for non-viral gene therapy?

08 September 2017 5,361 4 View

What's the reason i'm failing to install Autodock in windows 7?

04 May 2017 7,806 1 View

Is low validated protein model a problem for publication?

31 December 2016 4,394 6 View

Is it possible to conduct Agrobacterium mediated nitrogen fixing gene(nif gene) transfer into plant species?

Some bacteria naturally can utilize atmospheric nitrogen. My question is their any research is going to transfer nif gene into non-leguminous plant. Thanks in advance

07 August 2016 5,452 1 View

What are upstream & downstream effectors?

10 November 2015 1,197 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View