How to implement cytotoxicity test in microtiter plate?

I used 96 well and brine shrimp for cytotoxicity analysis of plant extract.After 48 hours hatching, the experiment was conducted. But the nauplli didn't survive even in the negative control....

31 December 2018 5,228 5 View

Is there any protocol to investigate Brine shrimp lethality test using microtiter plate?

I want to proceed cytotoxicity effect of plant extract which i found interesting against biofilm producing bacteria. Unfortunately, I've not much extract remain to implement test tube based...

11 December 2018 3,266 3 View

What is standard procedure to prepare plant extract stock solution for antimicrobial assay?

I'm following an article where they used 0.2% DMSO. I applied that but find out some problem as the dried extracts become sticky and don't dissolve at 100 mg/mL concentration. In another article,...

06 July 2018 1,707 4 View

What are the plant extracts that show both antibacterial and antibiofilm activity?

I need some papers( both research + review) where plant extracts been used both for both antibacterial and antibifilm candidate. Secondly, any database of secondary metabolites having...

03 April 2018 1,921 4 View

What's the protocol of TA cloning?

I'm looking for specific procedure of TA cloning. Best if someone provides this with published article.

01 February 2018 7,293 3 View

What's the standard procedure to prepare Agrobacterium stock solution?

In my lab, we lost the identification sign of Agrobacterium stock. So i tried to prepare stock by the following two procedures: 1. First i cultured Agrobacterium on solid YEP media and then...

10 November 2017 6,224 7 View

What's the reason i'm failing to install Autodock in windows 7?

04 May 2017 7,806 1 View

Is low validated protein model a problem for publication?

31 December 2016 4,394 6 View

Is it possible to conduct Agrobacterium mediated nitrogen fixing gene(nif gene) transfer into plant species?

Some bacteria naturally can utilize atmospheric nitrogen. My question is their any research is going to transfer nif gene into non-leguminous plant. Thanks in advance

07 August 2016 5,452 1 View

What are upstream & downstream effectors?

10 November 2015 1,197 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View