I'm following an article where they used 0.2% DMSO. I applied that but find out some problem as the dried extracts become sticky and don't dissolve at 100 mg/mL concentration. In another article, I find out using 10% DMSO to dissolve dried extract. Before applying
0.2% DMSO, I assayed their antimicrobial properties, which result 0.5% may have something like that.
Is their any laboratory standard to prepare stock solution of plant extract?
Make a stock of 200 mg/ml solution in DMSO with sonication. You can dilute further according to your need.
Not concentration dependent because of the variation in the nature and concentration of phytochemicals in pants. You can try different dilute concentrations to establish the Minimum Inhibitory Concentration of your extract.
We did covered extensively this subject in our forthcoming 3-volume set book of phytochemistry
http://appleacademicpress.com/phytochemistry-3-volume-set-volume-1-fundamentals-modern-techniques-and-applicationsbrvolume-2-pharmacognosy-nanomedicine-and-contemporary-issuesbrvolume-3-marine-sources-industrial-applications-and-recent-advances/9781771887625
It depends on the consituent of the plant.
If the plant contains for example flavonoids or lignans as antimicrobial agents. The total ethanolic extract concentrated and partitioned with ethyl acetate. The ethyl acetate layer dried and use for test. Using more than one extract give indication which comound is responsible for activity. So compare with total ethanolic extract
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