How to implement cytotoxicity test in microtiter plate?

I used 96 well and brine shrimp for cytotoxicity analysis of plant extract.After 48 hours hatching, the experiment was conducted. But the nauplli didn't survive even in the negative control....

31 December 2018 5,228 5 View

Is there any protocol to investigate Brine shrimp lethality test using microtiter plate?

I want to proceed cytotoxicity effect of plant extract which i found interesting against biofilm producing bacteria. Unfortunately, I've not much extract remain to implement test tube based...

11 December 2018 3,266 3 View

What is standard procedure to prepare plant extract stock solution for antimicrobial assay?

I'm following an article where they used 0.2% DMSO. I applied that but find out some problem as the dried extracts become sticky and don't dissolve at 100 mg/mL concentration. In another article,...

06 July 2018 1,707 4 View

What are the plant extracts that show both antibacterial and antibiofilm activity?

I need some papers( both research + review) where plant extracts been used both for both antibacterial and antibifilm candidate. Secondly, any database of secondary metabolites having...

03 April 2018 1,921 4 View

What's the protocol of TA cloning?

I'm looking for specific procedure of TA cloning. Best if someone provides this with published article.

01 February 2018 7,293 3 View

What's the standard procedure to prepare Agrobacterium stock solution?

In my lab, we lost the identification sign of Agrobacterium stock. So i tried to prepare stock by the following two procedures: 1. First i cultured Agrobacterium on solid YEP media and then...

10 November 2017 6,224 7 View

What are the nano-particles use for non-viral gene therapy?

08 September 2017 5,361 4 View

What's the reason i'm failing to install Autodock in windows 7?

04 May 2017 7,806 1 View

Is low validated protein model a problem for publication?

31 December 2016 4,394 6 View

What are upstream & downstream effectors?

10 November 2015 1,197 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Does anyone know of a predictable relationship beween surface gravity and atmospheric composition?

The attached file demonstrates that there is a predictable relationship between the structural density of a terrestrial body and its none-rotating surface gravity. Using Newton's gravity law of...

26 February 2021 2,317 3 View