I used 96 well and brine shrimp for cytotoxicity analysis of plant extract.After 48 hours hatching, the experiment was conducted. But the nauplli didn't survive even in the negative control. Crystal NaCl was used to make artificial sea water preparation.In addition, with or without covering 96 well plate resulting same thing. What's the reason of no survival nauplli in negative control?
Hi,
I am confused by your description. My definition of "positive control" in the context of cytotoxicity testing is something that is certain to kill your target cells. In that regard, I would absolutely expect the positive control to kill cells until you have diluted out the compound. Perhaps you started out with too high a concentration of chemical and/or solvent?
Oh, and as I was Googling around you might want to try following this protocol:
Article A MODIFIED MICROPLATE CYTOTOXICITY ASSAY WITH BRINE SHRIMP L...
Dear Niloy, what exactly was used as a negative control? I guess, that NaCl concentration could be too high?
Vilma Petrikaite I used 38g/L concentration of NaCl crystals and also 30 g/L NaCl
Dear Niloy Kumar Das
Marerial freshwater test material in seawater fails!!!
With Best Wishes
Peter Firbas
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