Niloy Kumar Das

18 Questions 20 Answers 0 Followers

Questions related from Niloy Kumar Das

How to implement cytotoxicity test in microtiter plate?

I used 96 well and brine shrimp for cytotoxicity analysis of plant extract.After 48 hours hatching, the experiment was conducted. But the nauplli didn't survive even in the negative control....

01 January 2019 5,303 5 View

Is there any protocol to investigate Brine shrimp lethality test using microtiter plate?

I want to proceed cytotoxicity effect of plant extract which i found interesting against biofilm producing bacteria. Unfortunately, I've not much extract remain to implement test tube based...

12 December 2018 3,350 3 View

What is standard procedure to prepare plant extract stock solution for antimicrobial assay?

I'm following an article where they used 0.2% DMSO. I applied that but find out some problem as the dried extracts become sticky and don't dissolve at 100 mg/mL concentration. In another article,...

07 July 2018 1,777 4 View

What are the plant extracts that show both antibacterial and antibiofilm activity?

I need some papers( both research + review) where plant extracts been used both for both antibacterial and antibifilm candidate. Secondly, any database of secondary metabolites having...

04 April 2018 1,981 4 View

What's the protocol of TA cloning?

I'm looking for specific procedure of TA cloning. Best if someone provides this with published article.

02 February 2018 7,362 3 View

What's the standard procedure to prepare Agrobacterium stock solution?

In my lab, we lost the identification sign of Agrobacterium stock. So i tried to prepare stock by the following two procedures: 1. First i cultured Agrobacterium on solid YEP media and then...

11 November 2017 6,301 7 View

What are the nano-particles use for non-viral gene therapy?

I need some articles presenting nanoparticles use for gene therapy in details. In addition, their synthesis and mechanism of action. Thanks in advance

09 September 2017 5,437 4 View

What's the reason i'm failing to install Autodock in windows 7?

I'm trying to install AutoDock 4.2.6 in windows 7 but whenever I write this command "C:\Program Files\The Scripps Research Institute\Autodock\autodock4.exe" at the command prompt, it show the...

05 May 2017 7,874 1 View

Is low validated protein model a problem for publication?

I'm working on a protein.I've found no suitable template for homology modelling for that protein. So, I used I-tasser for modelling and after validation, I got around 78-80% validation in...

01 January 2017 4,468 6 View

Is it possible to conduct Agrobacterium mediated nitrogen fixing gene(nif gene) transfer into plant species?

Some bacteria naturally can utilize atmospheric nitrogen. My question is their any research is going to transfer nif gene into non-leguminous plant. Thanks in advance

08 August 2016 5,526 1 View

What are upstream & downstream effectors?

In cell signalling process effectors play an important role.I found these two terms in several signalling book. What is the meaning of upstream& downstream effectors?

11 November 2015 1,434 3 View

What are the elements of cancer stem cells micro environment?

Currently i am researching about cancer stem cells micro-environment. I need some good papers(research) on this topic.Also therapeutic application targeting CSCs micro-environment. Thanks in...

11 November 2014 7,195 4 View

Does hormone/enzyme/specific protein have any effect on cancer stem cells for food consumption?

Cancer stem cells are the precursor of spreading cancer throughout the body. I need some article (research & review) where hormonal effect over cancer stem cells for food consumption...

10 October 2014 6,331 3 View

Conversion of c3 plant to c4 plant

Recently there is an ongoing research to convert c3 plant to c4 plant. IRRI, one of the pioneer doing this type of research. I need some good paper (both review&research) about this...

07 July 2014 2,815 6 View

I want to describe regeneration of cancer stem cells with the help of mathematics. Is this possible?

Just like we can elucidate bacterial growth by calculus.

02 February 2014 3,884 19 View

What type of methods should i use to identify the percentage of biochemical constituents in a crop sample?

I am thinking to implement a research work about the effect of biofertilizer on the biochemical constituents of a crop(e.g.maize). I need to know the overall methodology used on this type of...

09 September 2013 6,943 3 View

What is a biometric observation?

I was reading a research paper where i found the term.

09 September 2013 5,203 1 View

What is the composition of YEM medium?

I need details of the composition of YEM medium and, excluding rhizobia, which microbes are suitable to culture in this media?

08 August 2013 415 5 View