Format Mothur database to run through dada2?

I am looking to run illumina miseq data through dada2, but the limited identification of this gene means there are no pre-build databases. I have a database built in MOTHUR format, does anyone...

30 November 2020 8,228 3 View

How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

Has anyone used paraffin oil (liquid paraffin) instead of saturated alkane mixture in determining RI?

I would like to know if anyone has tried, or has any experience, in analyzing liquid paraffin with GC/MS and recording retention times. As hypothesized, this alkane mixture could maybe serve as a...

14 November 2019 8,547 3 View

Can I construct and ARDL model and test for cointegration with i(1), i(0) and i(2) variables?

Hi, I am currently doing a dissertation on demographic determinants of household savings in China during the One Child Policy, using time series. My variables are as follows: Household Savings- %...

21 July 2019 9,085 21 View

Rotating airfoil simulation - Fluent ansys - CL, CD?

Hello guys, I am in real need, I am working on my diploma thesis and the deadline is really close. I am having a bit of trouble with getting correct results of CL and CD from simulation over a...

13 April 2019 6,666 7 View

How do I increase the quality of the resulted video from Ansys Fluent simulation?

Hey! I am working on a flow analysis over a 2D airfoil.. I simulated the situation at different angles of attack, put it at 400 interations and everything works well in programme, although after...

09 April 2019 8,878 3 View

Is it possible to extract lift and drag curve from Ansys fluent?

Hey! I am currently working on flow analysis over 2D airfoil in Fluent ansys... I am new in this programme so after a day of hard work I managed to make the simulation according to the youtube...

09 April 2019 519 3 View

What cell culture contaminant turns media yellow but not turbid?

20 May 2015 4,389 11 View

Have anyone experienced that A549 and U251 cells grow in regular wavy lines in T75 flasks?

I have occasionally experienced that the two cell lines have grown in very regular wavy lines from the stopper to the opposite end of the flask. The cells seems to be okay except that they won't...

15 April 2015 9,034 2 View

Courses in Bioinformatics and NGS

Can anybody recommend specific courses in Bioinformatics and Next Generation Sequencing/ Deep Sequencing? What intense / short-term courses are available out there, and what are your experiences...

26 August 2013 9,904 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View