There are many online tools such as :

1- https://www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool

2- https://eurofinsgenomics.eu/en/ecom/tools/qpcr-assay-design/

3- doi: 10.1007/978-1-4939-2365-6_3.

Hi,

You can design probe and primer pair for QPCR via online tools. Here is a link:

https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/gene-expression-analysis-real-time-pcr-information/designing-taqman-gene-expression-assay.html

I hope this helps.

Thanks,

Alpana

Primer express software works good. But be careful with the operative system, doesn't work for Windows 10

Primer BLAST is one of the best and recently developed tools commonly used for picking primers. Here's a link...

https://www.ncbi.nlm.nih.gov/tools/primer-blast/

probe and primer pair can be design for qPCR via online tools. Here is a link:

https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/gene-expression-analysis-real-time-pcr-information/designing-taqman-gene-expression-assay.html

I think it would be helpful for you you can use Thermo Fischer tool also link given below

https://www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool