How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

Has anyone used paraffin oil (liquid paraffin) instead of saturated alkane mixture in determining RI?

I would like to know if anyone has tried, or has any experience, in analyzing liquid paraffin with GC/MS and recording retention times. As hypothesized, this alkane mixture could maybe serve as a...

14 November 2019 8,547 3 View

Can I construct and ARDL model and test for cointegration with i(1), i(0) and i(2) variables?

Hi, I am currently doing a dissertation on demographic determinants of household savings in China during the One Child Policy, using time series. My variables are as follows: Household Savings- %...

21 July 2019 9,085 21 View

Rotating airfoil simulation - Fluent ansys - CL, CD?

Hello guys, I am in real need, I am working on my diploma thesis and the deadline is really close. I am having a bit of trouble with getting correct results of CL and CD from simulation over a...

13 April 2019 6,666 7 View

How do I increase the quality of the resulted video from Ansys Fluent simulation?

Hey! I am working on a flow analysis over a 2D airfoil.. I simulated the situation at different angles of attack, put it at 400 interations and everything works well in programme, although after...

09 April 2019 8,878 3 View

Is it possible to extract lift and drag curve from Ansys fluent?

Hey! I am currently working on flow analysis over 2D airfoil in Fluent ansys... I am new in this programme so after a day of hard work I managed to make the simulation according to the youtube...

09 April 2019 519 3 View

Are there nonverbal coding schemes that focus specifically on leaders and followers in organizational settings?

19 April 2016 8,292 3 View

What causes problem with MC1061-DH5a plasmid compatibility?

We are frequently using plasmids with a certain backbone. Due to circumstances, some of these were maintained in Ecoli MC1061 and others in DH5a. Recently, we experienced problems with digests...

23 March 2016 4,739 3 View

Does anyone know of an estimate about ug total protein per OD600=1 of bacterial cells?

I am trying to find out if there are any reasonable guestimates out there on the amount of protein in a total bacterial lysate from a certain amount of cells. All I could find so far is estimates...

14 February 2016 6,172 5 View

How can I deal with different rRNA depletion results in RNAseq?

Hi, We have conducted a bacterial RNAseq using rRNA depletion on a NextSeq. Upon analysis, it seems some samples still have a significant amount of reads (5-40%) mapping to rRNA genes. How does...

19 September 2015 1,701 4 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View