Format Mothur database to run through dada2?

I am looking to run illumina miseq data through dada2, but the limited identification of this gene means there are no pre-build databases. I have a database built in MOTHUR format, does anyone...

30 November 2020 8,228 3 View

How do you co-culture astrocytes and neurons?

I am planning to co-culture primary astrocytes with immortalized neurons GT1-7 (both from mice). What is the best way to do this?

05 November 2020 5,040 4 View

Has anyone used paraffin oil (liquid paraffin) instead of saturated alkane mixture in determining RI?

I would like to know if anyone has tried, or has any experience, in analyzing liquid paraffin with GC/MS and recording retention times. As hypothesized, this alkane mixture could maybe serve as a...

14 November 2019 8,547 3 View

Can I construct and ARDL model and test for cointegration with i(1), i(0) and i(2) variables?

Hi, I am currently doing a dissertation on demographic determinants of household savings in China during the One Child Policy, using time series. My variables are as follows: Household Savings- %...

21 July 2019 9,085 21 View

Rotating airfoil simulation - Fluent ansys - CL, CD?

Hello guys, I am in real need, I am working on my diploma thesis and the deadline is really close. I am having a bit of trouble with getting correct results of CL and CD from simulation over a...

13 April 2019 6,666 7 View

How do I increase the quality of the resulted video from Ansys Fluent simulation?

Hey! I am working on a flow analysis over a 2D airfoil.. I simulated the situation at different angles of attack, put it at 400 interations and everything works well in programme, although after...

09 April 2019 8,878 3 View

Is it possible to extract lift and drag curve from Ansys fluent?

Hey! I am currently working on flow analysis over 2D airfoil in Fluent ansys... I am new in this programme so after a day of hard work I managed to make the simulation according to the youtube...

09 April 2019 519 3 View

What is the maximal value of OD at 600 nm measuring cell growth forreliable results?

05 May 2016 1,781 4 View

What cell culture contaminant turns media yellow but not turbid?

20 May 2015 4,389 11 View

Have anyone experienced that A549 and U251 cells grow in regular wavy lines in T75 flasks?

I have occasionally experienced that the two cell lines have grown in very regular wavy lines from the stopper to the opposite end of the flask. The cells seems to be okay except that they won't...

15 April 2015 9,034 2 View

Why does increasing the internal standard concentration (higher than the ULOQ) improves linearity in LC-MS/MS?

For a while, I have been experimenting with improving the linearity of my method. I have noticed that increasing my deuterium labeled (3x) internal standard to a concentration 2.5 times the ULOQ...

22 March 2020 2,892 7 View

Do deuterated compounds have a different pKa?

Do deuterated compounds have a different pKa? Specifically, I was wondering whether ethanol-D5 might have a different pKa than ethanol, and the mechanism behind this? Many thanks

31 December 2019 2,255 4 View

What is the phenomenon in which two deuterium atoms fuse together at room temperature?

I have heard in cold fusion , deuterium in heavy water fuse together to form Helium under some conditions. I want to know the mechanism behind it.

19 November 2019 7,399 4 View

What are the possible analysis can be taken from Energy spectrum of UV-Vis spectroscopy?

01 November 2019 6,789 3 View

How to replace hydrogen with deuterium in the input file using ADF software?

I am doing a calculation on deuterated system. So my question is, how I want to replace all the hydrogen atoms with a deuterium atoms in the input file. Is there any additional keywords?

19 June 2019 2,149 1 View

Why is TMS not added when Deuterium Oxide is used a solvent in HNMR spectroscopy?

Why is TMS not added in Deuterium Oxide in HNMR spectroscopy?

05 June 2019 6,403 3 View

What causes unexpected endothermic binding on ITC with sample in D2O?

04 May 2019 3,071 3 View

Imaging of biomolecules by AFM in liquid using D20 buffer?

Hello Everyone, I am really unable to measure in D2O buffer, for some reason. I even tried with higher forces, like around 250pN ( I usually need 50-80pN when using normal buffer) for my...

10 November 2018 8,924 0 View

Deuterium NMR method to observe quadrupolar splitting pattern in single-phase and multi-phase surfactant/water solutions?

I'm working on a method using Deuterium NMR (2HNMR) to observe different quadrupolar splitting patterns of single phase and multiphase surfactant/D20 solution (lamellar, hexagonal liquid...

06 July 2018 4,531 2 View

Optical dentisy(Od) of microbe using uv-vis?

Dear All I have checked OD(optical density) reading of microbe against dragon fruit peel extracted using uv vis. The wavelength use is 600nm. May I know as theorytically how this instrument get...

06 July 2018 1,764 0 View