Hi!
I an interested in culturing cervical kerationocytes and calcium levels in media are know to affect their cell growth. I recently obtained two KSFM for the same (one with Calcium and another without). I havent been able to get the exact calcium concentration for the 1x ksfm cat number-17005042 and would be happy if someone can help me with that. Also, what would be the ideal calcium concentration be that would suit keratinocyte growth?
Many Thanks.
Swetha
The extracellular concentration of Ca2+ should be in the span of 1.0-2.5 mM. You can try different concentrations of Ca2+ with steps of 0.5 mM and observe when cells are growing optimally. Maybe you can mix yore two medium 50/50 and then the concentration of Ca2+ should be half.
@Per Uhlen. Thank you. I was thinking of mixing the 2 media as well. But some literature says that the calcium concentration lesser than 1mM seems to work best.
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