Swetha Raghavan

9 Questions 9 Answers 0 Followers

Questions related from Swetha Raghavan

I am looking for a protocol to use for FACS based B and T cell immune profiling from PBMC and splenocytes from Mice?

Hi! I have to carry out a mouse B and T cell immune profiling FACS based assay from animals that have been immunized as a part of my study. I am new to immune profiling and I would be happy to get...

09 June 2021 6,354 4 View

Can somebody help me with method(s) to analyse and quantify oligonuleotides and protein interactions?

I am looking for an assay design that can help me analyse DNA (oligonucleotides) - protein interactions and provide some means to quantify these interactions. Any help or direction will be much...

15 July 2019 8,661 5 View

What is the calcium concentrations in KSFM and ideal calcium levels for cervical keratinocyte culture?

Hi! I an interested in culturing cervical kerationocytes and calcium levels in media are know to affect their cell growth. I recently obtained two KSFM for the same (one with Calcium and another...

15 May 2019 1,976 3 View

Mixed co culture experiment and MTT assays protocol?

Hello, I am trying to understand a co-culture experiment using different ratios of a cancer cell line and hematopoietic stem cell fraction in a mixed co culture. The ratios range from 1 to 1000...

12 October 2018 908 7 View

Can somebody help me with names of inhibitors/peptides against Progesterone Receptor B only and not the A form?

Hello, A part of my work is to understand the role of Progesterone receptor B role in a breast cancer related study and I have been looking for methods to differentiate the roles played by the A...

09 September 2016 6,434 2 View

How can you check whether estradiol treatment is working?

I have been using beta estradiol and checking for its effect on a particular gene promoter region. Treatment initially showed me a multifold increase when compared to promoter activity without...

19 June 2014 7,534 1 View

Can somebody help me with Methods/Assays to identify unknown protein binding to a specifice DNA sequence?

Usual DNA-protein interaction studies use ChIP/ arrays /EMSA to identify the interaction and in most cases we know the protein of interest. What can be done to identify unknown protein interacting...

17 June 2014 7,936 8 View

In vitro Kinase Assay-Band in negative control?

In my in vitro Kinase assay using purified proteins, I have been seeing a band even in my negative control (i.e without the Kinase) and it is almost of the same intensity as the protein of...

16 December 2013 2,247 3 View

HA-Ub expression failure observed after checking through western

I am trying to check the ubiquitination status of a protein by transfection of HA tagged ubiquitin followed by IP (for my protein) which in turn is followed by 24 hours serum starvation, before...

01 April 2013 4,168 1 View