What is the better option beta-NAD or NAD?

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How can we add External Lock-in Amplifier for Seiko SPA 400? I want to measure amplitude(1st harmonic ) vs DC bias sweep?

I need to add external lock-in amplifier but I am confused with connection.

29 January 2024 6,172 0 View

How we can calculate Vcpd after getting surface potential image ain KPFM ?

I have this image from surface potential how I can get CPD value from this data?

24 January 2024 1,795 4 View

Can anyone suggest an effective live cell plasma membrane dye for MDCK II TISSUE ?

Hi all, I'm working on MDCK II cell line where I grow the cells on PDMS substrate into a dense tissue (about 5mm diameter in size). I have been trying out different plasma membrane dyes for my...

14 January 2024 9,842 0 View

How can we calculate the amount of shelling precursor for the synthesis of core-shell nanoparticles to get particular shelling thickness?

We have the diameter of the core nanoparticles.

19 November 2023 3,113 3 View

What are the impurities of semaglutide?

What are the impurities formed during the synthesis and purification of semaglutide. kindly define,give their RRT and molecular mass also.

28 October 2023 8,130 2 View

How to obtain onset limit cycle oscillations in a Rijke tube?

with the boundary conditions of inlet as mass flow rate and outlet as pressure, the oscillation of pressure with flow rate is continuously diverging and it does not sustain limit cycle oscillation.

27 October 2023 1,385 0 View

How to decide the practical applicability of our prepared super-capacitor electrode material for specific application in portable electronics?

Hello, Researchgate community, I have one query regarding deciding whether our electrode material is suitable for practical energy storage applications. When we prepare and characterize...

22 October 2023 6,027 3 View

How can I decide ISI and jitter intervals for rapid stimulus presentation in fMRI picture memory encoding task?

The brain activity during individual images will be analyzed post-hoc for correctly recognized/ forgotten pictures during retention a few days later. Currently, we have 150 images; 3 seconds for...

17 September 2023 7,836 0 View

What exactly happens during the process of narrowing of C:N ratio?

How Carbon breaks, why N ratio remains same?

16 August 2023 9,957 4 View

How to decide the concentration need to induce oxidative stress in isolated mitochondria of rat brain by using either rotenone & sodium nitroprusside?

I'm trying to induce oxidative stress by using rotenone and SNP sodium nitroprusside in isolated mitochondria of rat brain. This is to study GSH assay from control (Mitochondria) group in...

11 June 2023 6,247 2 View

Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?

17 July 2024 935 1 View

Can I freeze a cell lysate supernatant for futuree NAD/NADH measurement?

Hello everyone! I need to measure NAD+/NADH levels using the NAD/NADH Quantification Kit (MAK037) from Sigma-Aldrich, and I´d like to know if I can first extract the supernatant containing...

06 March 2024 8,932 0 View

What is the best source of PK/LDH for low activity ATPases?

I am trying to establish a PK/LDH system to detect ATP turnover. We are having difficulty observing any measurable change in NADH fluorescence (we are not using the absorbance approach), and...

16 January 2024 2,680 1 View

What are the effects of pH on Fluorescence and Absorption of Flavins (Riboflavin, FMN, FAD) and NADH/NADPH in a Simple Aqueous Solution?

Dear all, I am interested in understanding the effects of pH on the spectrophotometric behavior of flavins (riboflavin, FMN, and FAD) and NADH/NADPH (including the oxidized form). Specifically, I...

14 October 2023 4,941 2 View

I am transforming my deletion strain of s pombe with my plasmids but the cells are turning red after spreading?

I am adding enough adenine in my plates

06 October 2023 365 5 View

Introduce mismatches in gRNA for base editing?

Hello everyone, I have five thymine (T) nucleotides in my gRNA, which I use for the adenine base editor. Due to PAM restrictions, I cannot use a different gRNA. I want to use AAV as a delivery...

14 August 2023 800 2 View

Which solvent should I use to dissolve NADH?

Can I use Tris buffer (pH 8.0) or deionized water?

13 June 2023 5,536 2 View

Is there any literature reporting NADH/NAD+ levels in different organelles or subcellular spaces?

23 March 2023 9,823 1 View

I am performing an enzyme assay where the output is NADH absorbance/fluorescence. Why would I see an increase even in my controls?

Hi there, I have been putting together an enzyme assay that measures the conversion of one sugar substrate into another sugar substrate of a reductase, with the goal of coupling enzyme 1's...

13 March 2023 5,089 2 View

How to Add methyl group to Adenin in RNA in Discovery studio or any other insilico method???

I wanted to add a methyl group in the adenine N6 position in the RNA for some docking and simulation studies. Is there any protocol or CODE for such editing to CH3 to the existing RNA ?? Is it...

12 March 2023 7,827 2 View