What is the better option beta-NAD or NAD?

More Madhura Kadam's questions See All

Whats the Alternate for cell recovery solution from matrigel ??

Hello, my work involves growing cells in the matrigel and for recovering cells from matrigel i usually use cell recovery solution from corning. As now i am curious to know if theres an alternate...

20 November 2020 1,148 3 View

Why do mammalian cells in culture undergo necrosis?

I am observing a large necrotic population in glioblastoma cells that I am culturing in 10% DMEM medium. In spite of changing all parameters of culturing and making sure of the sterilitu, the...

05 November 2020 9,280 3 View

How to purify polymer-peptide complex?

I am trying to conjugate a 12 amino acid peptide (MW 1565Da) to PLGA-PEG-maleimide copolymer (MW: 35097Da). The conjugation reaction takes place in ACN: DMF as a solvent. Can someone please...

19 October 2020 636 5 View

Can we simulate blockchain network in NS#?

I am new to ns3 and I want to know if it is possible to simulate blockchain network on NS3 and if yes, can anyone share some links to tutorials that would be helpful for this. It would be helpful...

08 March 2020 7,384 1 View

Modeling of benzene recovery process by extractive distillation?

I am trying to model a Benzene recovery process by extractive distillation in Aspen plus. The calculation type chosen for the radfrac column is 'equilibrium' with the tray efficiency of 75%. i...

04 February 2020 9,279 3 View

Can i grow hacat and L929 cells with dmem high glucose + 4mM glutamine?

I am gonna grow hacat and L929 cells and can i grow these cell line with dmem high glucose+ 4mM L glutamine? Which is more preferable 2mM or 4mM glutamine? Thanks in advance.

09 March 2019 4,028 3 View

Can I use DMEM high glucose + 4M glutamine in culturing the L929 cell line??

I am going to grow L929 cells for scratch assay and i have been told that i need to grow them on DMEM low glucose with 2M glutamine and 10% fbs . can i grow it on high glucose with 4M glutamine...

27 February 2019 6,480 2 View

How to add randomness to a channel using Matlab simulations?

I have to simulate multipath fading channels and add randomness to it.

21 September 2018 6,377 2 View

Can someone give me suggestion over following choice experiment as to how I can place the variables in a right way?

Please suggest me how can I better place the variables in the following choice experiment. CE1. Please look at the following scenario and select the level of self-driving car you would consider...

03 April 2018 1,864 2 View

How to get rid of bubbles in plastic film?

Hi, What could be the reasons that a film prepared by casting a film forming solution on petri plate gets bubbles into it? How can it be prevented? Thanks, Ashish

08 February 2018 3,804 4 View

How can I force Gaussian to search the exactly point group of my molecule?

Hi, I want to know the point group of my molecule "adenine-thymine" , it is composed by 2 molecules ( thymine and adenine) and both of them belong to Cs point group. What can i do ?? help please.

02 December 2020 7,091 3 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

What are the necessary parameters for measuring GAPDH NADH production over time in a UV transparent 96-well plate?

I am looking to measure NADH production at 340 nm by GAPDH (from crude protein extract) over time in a UV transparent 96-well plate. So far I am unable to detect any signal and I was wondering if...

12 February 2020 7,376 2 View

How bad is this overlap primer with 2 sets of sextet adenine?

Neb Builder suggested this primer to me for PCR overlap ligation, and I appear to be stuck with it. At least it isn't 6 guanines! How much trouble do you think this bad forward primer could...

31 December 2019 3,921 3 View

What can be used in SOD assay in place of NADH?

I have to do SOD assay using phenazine methosulphate and sodium pyrophosphate buffer on rat tissues. The protocol says that NADH is required to start the reaction but I don't have that. I want to...

31 December 2019 8,104 2 View

Does peptone contain Adenine and uracil Its urgent please reply ASAP?

I am unable to find an exact selection media where I can see the phenotype of adenine mutants from sick to cure after transformation on a plate So, my question is about peptone composition and...

11 December 2019 7,370 4 View

Does peptone contain Adenine and uracil Its urgent please reply ASAP?

I am unable to find an exact selection media where I can see the phenotype of adenine mutants from sick to cure after transformation on a plate So, my question is about peptone composition and...

06 December 2019 3,761 4 View

Can tissue samples be deproteinized for measurement of NAPD/NADPH with 4%w/v sulfosalycilic acid?

How will 4% w/v SSA interfere with the NADP/NADPH ratio? Can this method of deproteinization be used as an alternative to 10 kDa cut-off spin columns in order to measure NADP/NADPH ratio?

10 November 2019 3,514 3 View

How to plan a classical enzyme-substrate reaction ???

Hello everyone! I am mostly a structural biologist who did not get a chance yet to delve into a classical biochemical enzyme-substrate reaction. So as things would have it, I will have to perform...

15 October 2019 7,411 1 View

How to plan a classical enzyme-substrate reaction ???

Hello everyone! I am mostly a structural biologist who did not get a chance yet to delve into a classical biochemical enzyme-substrate reaction. So as things would have it, I will have to perform...

09 October 2019 5,119 1 View