I am doing a cell culturing experiment in a fluid flow chamber made of PDMS material. I was wondering how to sterilize the pipes and the flow chamber before/after the experiment, in case I want to use the same chamber for the second and third biological repeats?
The primary benefit of PDMS devices are that they are cheap, easy to produce and disposability. They are not meant to be reused.
Hi,
We used devices repeatedly made up of PDMS as well as other materials with pathogenic bacteria. As long as the materials are Autoclaveable there is no problem in reusing them. Infact the current scenario of having sustainable development contains inherent clause of showing how we can use the same devices for longer periods with use of minimal processing. Apart form that, technically PDMS has shown to provide good results as long as the devices made from it are sterilized meticulously. It all percolates down to how good your skills are while handling cell cultures!!!
Regards
Amit
Amit Morarka thank you very much for answering. could you please provide details of the setting that you normally use in autoclaving PDMS? also do you autoclave it with liquid or only heat?
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