14 Questions 13 Answers 0 Followers
Questions related from Mustafa Nile
I am running SDS-Page western blot using 10% acrylamide gels. However, my samples are not migrating more than 55 kDa. The bands are not defined. I am using 4x Laemmli buffer with LDS from Biorad....
23 April 2024 7,348 11 View
Is there any available online tool, which can be used to do primer-check for primer location in terms of Exon/Intron binding sites? Similar In silico website, which is currently not working is...
22 February 2023 6,384 3 View
I have noticed that there are single microscopic slide/slip chambers (Cytodyne, Flexflow, IBIDI) and many studies have used these chambers. I wondered how it is possible to have more robust data...
26 September 2022 8,997 1 View
I would like to know how to coat a specific area (for example: the center of the slide only) with collagen typ1? Should I coat the whole slide then use a blade to scrape the undesired coated area?
20 January 2022 854 3 View
I have been searching for the equation of calculating the pressure inside a rectangular pipe using volumetric flow rate but all I am finding is the Hagen–Poiseuille equation that calculates the...
05 January 2022 1,143 2 View
I was wondering where to find the viscosity of cell culturing media under 37C, As I am planning to do a microfluidics experiment using DMEM (high and low glucose) and KSFM at 37C temperature?
19 November 2021 910 1 View
What is the best way to bond PTFE membrane to PDMS material in order to use it as a bubble trap in a microfluidic device?
26 October 2021 547 4 View
Hello everyone, I tried to export an .stl file of a 3D designed pipe adapter with threads in AutoCad 2022 Software, however, the threads were missing. I tried multiple times but all of my attempts...
19 October 2021 5,729 3 View
I will be doing a fluid flow experiment on cells and I was wondering what are the best, yet affordable peristaltic pump and fluid-flow meter/shear stress detector available in the market?
03 October 2021 5,050 3 View
How to connect Autocad Software from a laptop to a 3D printing machine or a milling machine, to execute a 3D design?
20 September 2021 1,374 4 View
I am doing a cell culturing experiment in a fluid flow chamber made of PDMS material. I was wondering how to sterilize the pipes and the flow chamber before/after the experiment, in case I want to...
11 August 2021 5,238 3 View
My question is regarding how to make a custom-made culturing flasks (micro-fluidics) using a mold and flowable material that is similar to resin? what material is the most suitable in terms of...
20 July 2021 880 3 View
Does anyone know an online tool similar to "projects.insilico.us/SpliceCenter/PrimerCheck.jsp" to determine primers location in terms of exon/intron binding sites? any alternative available? Thanks
01 January 1970 1,678 1 View
How long does fluid flow within bone lacunae last when applying a constant force? Is it going to last for a few seconds till fluid flow equilibrium occurs? During jogging, walking and jumping...
01 January 1970 7,522 0 View
