What is the best way to inactivate HIV in the supernatans of an NK-CD4 co-culture?

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Corning MITO+ Serum extender??

Hello, Does anyone have a substitute for Corning® MITO+ Serum Extender ? Or have the specifications for the compounds in it? It seems to be on back ordered forever. Please help. julie

16 April 2023 2,164 4 View

Where to find alab-size impact crusher or cone cursher that would be able to produce aggregates up to 20 mm?

We would like to produce concrete aggregates that have a similar shape as industrially produced aggregates to further investigate recycling in new concrete. In industry, they use a jaw crusher...

19 January 2021 701 3 View

Oldenburg Burnout Inventory in Dutch?

Dear colleagues, I am searching for a Dutch version of the Oldenburg Burnout Inventory. Who can provide me a PDF version or a link to an internet source? Thanks in advance. Best regards, Jef...

19 October 2018 5,757 2 View

How do I add the redox couple Pb/Pb2+ to visual minteq?

I'm trying to model the solubility of lead in different conditions. However, I currently find no way to include the formation (and precipitation) of Pb°

03 June 2018 8,632 2 View

Specific application of Al6061/SiC MMCs?

We know that MMCs are used in automotive, aerospace, defence industry, but I want to know In which parts we use Al6061/SiC cposite, if possible with reference.

04 May 2018 3,657 9 View

Application of Al6063/SiC, Al6065/SiC?

For my project I am little bit confuse about selection of Aluminium grade. I want an unique composition which have more industrial use and also available in market.

18 July 2017 3,765 0 View

At which viral load should the CD56neg NK cell subset be significantly present in HIV patients ?

We try to isolate CD56neg NK cells from HIV patients, but the presence of this NK cell subset seems to be correlated with the viral load so I wondered from which viral load you can assure the...

20 October 2015 8,615 1 View

How much plasma do I need for Next Generation Sequencing of miRNAs from humans?

I've seen information where 100-200 ul of plasma was used for miRNA RT-PCR, but if I wanted to do next generation sequencing(NGS) on miRNAs from human plasma, how much plasma do I need to start...

22 September 2014 9,235 8 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View