I am working on differentiation of BM MSCs into insulin producing cells, we used to separate Nestin +ve from BM MSCs using a magnetic nanopartice kit and nestin antibody, but the purity is not high, so what would be the best accurate way to isolate these Nestin positive cells?
FACS method is appropriate for cell sorting but MACS isn't appropriate method because nestin isn't a surface marker
Maryam nazm bojnordi: thanks for your reply, but i wonder if FACS method is suitable for culture of the isolated cells..in term of sterility and viability? as i need to isolate nestin positive cells and then culture them for further differentiation ...and i will be grateful if you provide me with a reference reporting that nestin is not a surface marker...
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line