Sawsan Mohammed Elhalawani

6 Questions 5 Answers 0 Followers

Questions related from Sawsan Mohammed Elhalawani

Can i use the Qiagen RNeasy Mini Kit used for RNA extraction for cells and tissues for the extraction of RNA from MSCs derived exosomes?

If it is applicable, What should be the recommended concentration of exosoemms as the starting sample?

29 October 2022 2,767 3 View

Have anyone ever experienced any islets regeneration in the pancreas of diabetic (STZ) male S.D rat?

Hi, is it possible that pancreatic islets of male S.D rats could regenerate after induction of diabetes using 45 mg/Kg STZ?

27 December 2017 7,780 2 View

How to over come the cross reactivity of rat insulin ELISA kit with human insulin?

Hi every body, currently i'm working in a diabetes project, we are culturing human MSc in diabetic S.D rats, and i have to measure both human and rat serum insulin.. but, i am facing a problem...

24 September 2017 3,263 2 View

Does anyone know the best protocol for differentiation of adult stem cells into definitive endoderm?

most of protocols for the differentiation of stem cells into endoderm involve factors like Activin-A, CHIR, Wartmmanin... but i would like to know if anyone has experience in this type of...

22 March 2016 8,080 0 View

What is the best way for the isolation of high purity Nestin positive cells?

I am working on differentiation of BM MSCs into insulin producing cells, we used to separate Nestin +ve from BM MSCs using a magnetic nanopartice kit and nestin antibody, but the purity is not...

17 July 2014 9,917 2 View

Can anyone tell me where I can send a sample for proteomic analysis?

If I have a sample of rat pancreatic extract after 2 days of pancreatic surgical injury, how can I know the total protein profile in this pancreatic extract? Or where can I send a sample to get...

14 July 2014 3,436 3 View