Is there any research paper about integrating mobile application like google classroom in teaching?

This is to support or serve as a guide for my thesis regarding 21st century teaching strategies. Thankyou so much in advance!

07 December 2020 8,440 4 View

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30 September 2020 7,720 2 View

How should the SWAT-CUP objective function results be interpreted?

I am not sure how to interpret the objective function results in SWAT-CUP. I have six observed variables (two streamflow sites, and four soil moisture sites) with R-squared results ranging from...

29 June 2020 3,652 3 View

Is there a way to conduct robust variance estimation (RVE) using the Comprehensive Meta-Analysis (CMA) program?

I've averaged multiple effect sizes within the same study to a single mean effect size per study, however I know this method underestimates the study's standard error and overestimates study...

04 February 2020 4,738 2 View

How do you lyse small cells (10 microns) without disrupting the mitochondria?

We consistently isolate intact/functional mitochondria from liver and heart tissue using manual homogenization (to disrupt the cell membrane) followed by differential centrifugation. I am now...

04 November 2019 9,279 1 View

What impact does burnt vegetation particulates have upon raainfall and local climate -does it reduce rainfall?

I am no longer at an Academic institution so cannot access many research articles such as Langmann B, Duncan B, Texter C, Trentmann J, van der Werf, GR (2009) Vegetation fire emissions and...

02 October 2019 5,352 3 View

What are specific dendritic cells and macrophages markers for mouse adipose tissue??

I will analyse stromal-vascular fraction of adipose tissue by FACS. The panel of antibodies could be F4/80, Cd11b, Cd45.2, Cd11c and Cd301. The macrophages are f4/80 and Cd11b positive, and Cd11c...

07 May 2019 9,687 1 View

Is Bushnell Haas (BH) Medium appropriate for enrichment of hydrocarbon-degrading microorganisms isolated from marine environment?

The composition of the BH medium is as follows: MgSO4 - 0.2g/L CaCl2 - 0.02g/L KH2PO4 - 1g/L K2HPO4 - 1g/L (NH4)2SO4 - 1g/L and FeCl3 - 0.05g/L Thank you!

06 May 2019 4,680 5 View

Can someone share the syntax for splitting a large SPSS file and saving the output files as CSV or XLS?

I am trying to split a file of over 200,000 cases by a site ID. I need to get these files into excel. I do not want to have to split the SPSS file into 280 smaller files then manually save each as...

16 April 2019 2,903 3 View

How to count cells that are both GFP and dapi positive?

I am doing a competition grow assay where GFP+ keratinocytes and mCherry+ kerationcytes are plated together, allowed to grow and passed multiple times. At each passage, cells are plated onto a...

04 September 2018 4,005 5 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View