When staining for tetramer positive cells, what is the best order when you also need a fixable viability dye? ie. Live/dead >> tetramer >> surface cocktail or tetramer >> live/dead >> surface cocktail. And can any of the staining steps be combined?
Hi guys, I am running WB on JESS (ProteinSimple) targeting p65 (NFkB) and we are, for some reason, detecting only a 46kDa band in the liver tissue. This is the cytosolic portion of the...
16 June 2024 4,759 0 View
I am having trouble narrowing down the social justice framework of Physician Assisted suicide. thank you for your time. Nicole
09 June 2024 5,836 2 View
I'd like to measure trans fatty acids in industrial foods. What method of analysis would you recommend? I've heard of microwave-assisted extraction techniques for processing samples. My lab only...
30 April 2024 5,269 1 View
Student has HFA and is a 15 year old male
30 April 2024 3,887 2 View
Upon reading and understanding the article entitled “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar Reddy Kasarla and Laxmi Pathak, it was highlighted that specimen...
10 April 2024 4,653 3 View
I'm eager for insights concerning the impact on sample quality and data integrity in the clinical laboratory.
11 March 2024 4,081 1 View
Can Rasch analysis indicate which items belong to a certain category? I am trying to validate a survey about anxiety. The Likert scale is from 1 to 6 for each of the 17 items. My goal is to...
22 February 2024 6,376 3 View
I keep getting confused that if it's true experimental design, who would answer the pre and post test?
06 February 2024 8,577 1 View
I am conducting an on-going research study and I am evaluating the performance of consortium of bacteria as a biocatalyst in a microbial fuel cell. There's some misunderstanding between me and the...
05 February 2024 8,252 1 View
I am looking for other alternative methods to measure the electrolytes aforementioned since other studies claim that Indirect ISE can induce error in its results due to its dilution step.
24 January 2024 7,519 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?
03 August 2024 607 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View
As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...
31 July 2024 6,484 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View