Also, after extraction, how do I ensure that I got aflatoxins? I cannot use chloroform since its banned. I also don't have access to UV.
thank u Dr. Naqvi. ideally, we would of course use HPLC for analysis but unfortunately, we don't have the machine and its very expensive. can u suggest an alternative?
If you do not have then you can perform thin layer chromatography but you should have standard of aflatoxin
I agree with Dr. Naqvi, you could use TLC, but if you don't have a pure sample of aflatoxin you could consider buying a detection kit for aflatoxins. Maybe an ELISA kit could be helpful.
That makes good sense. Thank you Dr. Naqvi and Dr. Ramming! Have any of you tried TLC of aflatoxins? Maybe you can suggest an effective developing solvent. Also, can TLC only be used if I have pure toxin?
The "pure" sample mentioned which was meant for a positive control. If you are thinking about using ELISA, you should take a look at the aflatoxin clean-up columns from r-biopharm.
The best solvent to extract them is acetonitrile but is expensive. Another option is methanol which is the standard solvent used. If you don't have UV or HPLC you can use fluorescence readers like the ones from VICAM.
Thank you for the advice Dr. Alicia and Dr. Roman.
Dr. Amr, I am actually interested in aflatoxin reduction through biocontrol. But we have a group that looks at bioactive compounds of plants and fungi.
That would be a good idea Dr. Mohammed but I don't know how to transfer a question. An editor transferred my question to this topic.
Johnson & Abu-Shumays (1977) using to extract them are 40ml (8:2 V/V) metanol water with 20 grams of rice
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