I need to prepare liposomes from DPPC/DOPC/Cholesterol mixture. From your experience what is the best ration for having higher encapsulation efficiency?
Greetings,
We have used DPPC:DCP:Chol (7:2:1 ratio) in the preparation of liposomes / nanoliposomes. The combination we used is slightly different than yours, although we obtained good results with respect of stability of the vesicles, E.E., drug release, etc.
The following articles mention exactly your combination (DPPC/DOPC/Chol), check them out:
Reproduction of a Three-Component (DPPC/DOPC/Cholesterol ...
www.cell.com/biophysj/abstract/S0006-3495(16)31473-4
by C Montour - 2017
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Phase Diagram using Coarse Grained Molecular Dynamics
www.cell.com/biophysj/pdf/S0006-3495(16)31473-4.pdf
by C Montour - 2017
Feb 12, 2017 - the oft-used CHARMM C36 lipid force field. 378-Pos Board B143. Reproduction of a Three-Component (DPPC/DOPC/Cholesterol) Phase.
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The Mechanism of Collapse of Heterogeneous Lipid Monolayers
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
by S Baoukina - 2014 - Cited by 17 - Related articles
The monolayers of 5:3:4 DPPC/DOPC/cholesterol at 290 K formed networklike Lo domains separating Ld-phase regions (Fig. 1 c). This monolayer had a nonflat ...
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The partition of cholesterol between ordered and fluid bilayers of ...
www.sciencedirect.com/science/article/pii/S0005273607002854
by L Chen - 2007 - Cited by 42 - Related articles
An X-ray scattering intensity pattern recorded from a ternary mixture consisting of 1:1:0.5 mole ratioDPPC:DOPC:cholesterol (20 mol% cholesterol) shows a ...
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Advances in Planar Lipid Bilayers and Liposomes
https://books.google.com.au/books?isbn=0123810140
Ales Iglic - 2010 - Science
Time resolved synchrotron small angle X-ray scattering (TRSAXS) has been used to study the kinetics of phase separation in DPPC–DOPC– cholesterol ...
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Use of fluorescence to determine the effects of cholesterol on lipid ...
www.jlr.org/content/49/6/1202.full
by BM Stott - 2008 - Cited by 29 - Related articles
Feb 25, 2008 - DPPC/DOPC/cholesterol phase map. The experiments shown in Figs. 1–5 were repeated with liposomes containing equimolar mixtures of ...
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You are most welcome Ahmad.
I wish you all the best in your project and will be happy if I can help you in any way possible.
Dear Dr. Mozafari,
I need to prepare liposomes of DSPC/DPPC/DSPE-PEG , Which ratio of lipids is the best for higher encapsulation effeciency for a highly lipophilic drug?
Thank you
Dear Dr Dayyih,
I just saw your question here, sorry for late reply. Unezaki et al. used a molar ratio of:
1: 9: 0.61 for DSPC:DPPC:DSPE-PEG liposomes.
S. Unezaki, K. Maruyama, N. Takahashi, M. Koyama, T. Yuda, A. Suginaka, M. Iwatsuru. Pharm. Res., 11 (1994), pp. 1180-1185
as mentioned in this paper:
Improved retention of idarubicin after intravenous injection obtained ...
https://www.sciencedirect.com/science/article/pii/S0005273602003450by N Dos Santos - 2002
Have a look at Santos paper, it also explains other lipid combinations and molar ratios.
How i can calculate the amount of soya PC and Cholesterol for various ratio. Please explain the calculation.
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