Which is better for cell lysis preparation for ELISA: Triton X100 or Tween 20? What is the percentage that needs to be used?

I am preparing cell samples for ELISA to measure intracellular (total) protein expression. I do not want to extract the proteins as this is not compatible with the kit we have available. I want to...

15 March 2017 1,729 3 View

How effective are global health interventions in developing countries?

I am researching for effective models and approaches to deliver effective and sustainable improvements. Consulting by experts to identify and solve problems, projects to deliver services to the...

23 June 2016 6,312 6 View

Simulation /wear Analysis of Turbine Blades ?

17 January 2016 6,631 3 View

Is nuclease free water required for DNA quantification? If so, what are the repercussions of not using it?

Is nuclease free water required for DNA quantification? If so, what are the repercussions of not using it?

05 January 2016 5,407 3 View

Does anyone know how to set the Custom Field Functions in FLUENT?

Hello everyone, I have few questions : I'm newbie in ANSYS Fluent (CFD simulation).I want to make a simulation of Pool Boiling using PCB as a heater.Here is my geometry. I set up the heater's...

22 November 2015 3,947 3 View

What is the best vector for a TetON system with a bidirectional promoter?

I cloned 2 genes of my interest into the pBI-4 vector, so that I could activate the expression of both genes at the same time. However, the construct is quite leaky, so that I get expression of...

04 June 2014 2,144 2 View

What is the best way to transfect HUVECs?

I tried Metafectene, peqFECT and Fugene. Nothing worked so far.

02 October 2013 1,371 11 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

QTL, recombination frection?

22 February 2021 2,588 2 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View