5 Questions 34 Answers 0 Followers
Questions related from Francisco Javier Rodríguez Baena
I have been trying to extract RNA from mouse spleen for a while and the results it's always the same, I end it up with the whole RNA degraded. I use the RNA-II extraction kit from Macherey-Nagel...
20 February 2016 7,312 4 View
I am isolating murine macrophages from the bone marrow (BMDM). I was able to isolate them and grow them in culture using m-CSF. They grow nicely and I have checked by flow cytometry that about the...
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I would like to isolate macrophages from tissues as well as different tumors from mice and I can not find an appropriate protocol for it.
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I am looking for the easiest methodology to obtain a good number of BMDM from my mice set. I have been told (also read in papers) to obtain the bone marrow from the leg bones by cutting the edges...
28 December 2014 3,621 29 View
I can't find the original one anywhere else and I would like to have it
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