Elvira Mass

3 Questions 6 Answers 0 Followers

Questions related from Elvira Mass

What is the best protocol to induce Cre recombinase via tamoxifen in bone marrow derived macrophages?

Using 4-OHT for 4 days, we could (only) reach up to 5% recombination. But this treatment led also to a high apoptosis rate.

04 December 2014 2,037 4 View

What is the best vector for a TetON system with a bidirectional promoter?

I cloned 2 genes of my interest into the pBI-4 vector, so that I could activate the expression of both genes at the same time. However, the construct is quite leaky, so that I get expression of...

05 June 2014 2,197 2 View

What is the best way to transfect HUVECs?

I tried Metafectene, peqFECT and Fugene. Nothing worked so far.

03 October 2013 1,426 11 View