Elvira Mass

3 Questions 6 Answers 0 Followers

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What is the best protocol to induce Cre recombinase via tamoxifen in bone marrow derived macrophages?

Using 4-OHT for 4 days, we could (only) reach up to 5% recombination. But this treatment led also to a high apoptosis rate.

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What is the best vector for a TetON system with a bidirectional promoter?

I cloned 2 genes of my interest into the pBI-4 vector, so that I could activate the expression of both genes at the same time. However, the construct is quite leaky, so that I get expression of...

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What is the best way to transfect HUVECs?

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