I'm using commercially available kit and plastic plates, but I heard that maybe wells coated with polylysine would provide better results? Does anyone have any experience with osteogenic differentiation on plates coated with polylysine etc?
Hi Patrycija, these are our 2 established protocols for osteogenic differentiation, you need to change them slightly to adapt to your needs but I have been using them for a long time and they work really well in our lab. hope they help you
Thank you very much for protocols. It will be very useful in my laboratory work.
Hi Patrycja,
I've attached a very simple assay to quantify osteogenic differentiation. I have used this successfully with adipose derived MSC.
Cheers,
Chris
Thank you Christopher for your protocol.
Could you answer me? What is the KO medium that used for dissolving BGP?
Thank you very much
Antonio M Spadaccino For the protocols detailed above, would they work for the osteogenic differentiation of bone marrow derived MSCs as well as adipose derived MSCs? Many thanks!
I think so, normally the protocol works very well with bone marrow derived sc so i don't see why should not work from adipose derived sc. the only thing i would do is try anyway, the reagents are quite easily found in any labs. The only thing i remember was the difficulty of isolating sc from adipose tissue and not the differentiation. I abandoned it at the time because was very low yielding and did not allow to do many studies from the lack of sizeable amount of cells found
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