Sarah K Stewart

4 Questions 5 Answers 0 Followers

Questions related from Sarah K Stewart

Does anyone have a protocol for freezing bone marrow aspirate concentrate (BMAC)?

I am collecting BMAC samples from human subjects, and plan to analyse the MSC count from each sample and assess growth factors for ELISA. However, I can only obtain the samples in a dripfeed...

07 November 2019 2,454 0 View

How do I expand MSCs from Bone Marrow Aspirate Concentrate (BMAC)?

Hi :) I'm looking to expand MSCs from human BMAC, and then to subsequently characterise them. A lot of papers talk about 'washing' the BMAC with PBS prior to resuspending in media and plating....

20 June 2019 8,811 0 View

Can anyone recommend a osteogenic medium for bone marrow derived MSCs?

Can anyone recommend a osteogenic medium for bone marrow derived MSCs? I have two conflicting protocols from colleagues with differing concentrations of reagents, and not sure which one to use. I...

08 April 2019 10,000 0 View

Does anyone have a reliable protocol for thawing bone marrow derived mesenchymal stem cells?

Hi all, I'm a PhD student using bone marrow derived MSCs bought from a commercial supplier. The instructions don't come with a protocol for thawing them, so am using a protocol that I use for...

19 February 2019 5,334 10 View