Does anyone have experience with PCR MP (Melting Profile) on Candida albicans strains? I performed digestion and ligation reactions on EURx reagents (HindIII, T4 DNA ligase)? I can't get the genetic material of the mushrooms cut up.
Hi, We have been struggling to establish the A20 (ATCC TIB-208) mouse B-cell lymphoma subcutaneously injected into the right flank. However, in our case, this model varies a lot between separate...
04 October 2023 1,105 0 View
I want to do a cascade. In the first step I use Grignard reagent and magnesium ion from it blocks my second step. How to quench it or bond with something else than oxygen? [EDIT] I know how to...
23 May 2021 3,880 5 View
Welcome everyone! In my research I focus on comparing Staphylococcus aureus genes to the reference gene - gmk. In qPCR I’m using relative quantification and the following formula: ∆Ct (target)=...
07 August 2018 8,021 0 View
Hi! I have a problem with the analysis in LightCycler 480 Software. I prepared the two-step cycling experiment with the following conditions: 95C 15s, 60C 1 min (see the picture below). I based on...
06 March 2018 6,884 7 View
I want to study how the ideologies and rhetoric of far right parties in Poland have developed in the last 20 years due to Globalization processes. Which research methods would be the most suitable?
16 August 2015 4,675 16 View
I'm using commercially available kit and plastic plates, but I heard that maybe wells coated with polylysine would provide better results? Does anyone have any experience with osteogenic...
13 May 2014 4,614 9 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View