What is the best procedure to quantify DNA damage on paraffin section stained with Feulgen?
I'm looking for DNA damage on testicular seminiferous tubes.
You could try immuno-staining for gamma-H2AX and count foci, immuno-staining for RAD51 is another option.
I've been trying to perform the warthin-Stary protocol for Leptospirosis in mice kidney. However, the positivity desapears overnight - Is this normal? I already tried the oven and the microwave...
09 October 2018 4,374 0 View
Could anyone name a full protocol for TNF-alpha immunohistochemistry for paraffin sections and antibody reference and brand? Thanks in advance
09 October 2017 1,401 4 View
I'm considering to buy a cassette marker for histological cassettes and slides. What are the best options? Laser or Ink? And what are the best machines out there, both affordable and with quality.
03 April 2014 10,060 0 View
What are the best analysis you can perform on slides with TUNEL fluorometric test? Qualitative or quantitative analysis?
09 October 2013 6,702 1 View
I have mice in storage in formaldehyde 4%, and I would like to study the amount of fluorine existent in their teeth. Does long fixation time affect the amount of fluorine or calcium on the mouse...
08 September 2013 1,070 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?
02 March 2021 1,493 4 View