I've been trying to perform the warthin-Stary protocol for Leptospirosis in mice kidney.
However, the positivity desapears overnight - Is this normal?
I already tried the oven and the microwave oven...
Could anyone name a full protocol for TNF-alpha immunohistochemistry for paraffin sections and antibody reference and brand? Thanks in advance
09 October 2017 1,566 4 View
I'm considering to buy a cassette marker for histological cassettes and slides. What are the best options? Laser or Ink? And what are the best machines out there, both affordable and with quality.
03 April 2014 10,222 0 View
What are the best analysis you can perform on slides with TUNEL fluorometric test? Qualitative or quantitative analysis?
09 October 2013 6,851 1 View
I have mice in storage in formaldehyde 4%, and I would like to study the amount of fluorine existent in their teeth. Does long fixation time affect the amount of fluorine or calcium on the mouse...
08 September 2013 1,247 0 View
What is the best procedure to quantify DNA damage on paraffin section stained with Feulgen? I'm looking for DNA damage on testicular seminiferous tubes.
06 July 2013 1,542 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
29 July 2024 5,622 3 View