I'm considering to buy a cassette marker for histological cassettes and slides. What are the best options? Laser or Ink? And what are the best machines out there, both affordable and with quality.
I've been trying to perform the warthin-Stary protocol for Leptospirosis in mice kidney. However, the positivity desapears overnight - Is this normal? I already tried the oven and the microwave...
09 October 2018 4,523 0 View
Could anyone name a full protocol for TNF-alpha immunohistochemistry for paraffin sections and antibody reference and brand? Thanks in advance
09 October 2017 1,566 4 View
What are the best analysis you can perform on slides with TUNEL fluorometric test? Qualitative or quantitative analysis?
09 October 2013 6,851 1 View
I have mice in storage in formaldehyde 4%, and I would like to study the amount of fluorine existent in their teeth. Does long fixation time affect the amount of fluorine or calcium on the mouse...
08 September 2013 1,247 0 View
What is the best procedure to quantify DNA damage on paraffin section stained with Feulgen? I'm looking for DNA damage on testicular seminiferous tubes.
06 July 2013 1,542 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Does anyone have any case studies on the Environmental Impact of Organic Manure Production Unit? Environmental Impact of a Organic Manure Production Unit Facility is what I studied but...
16 July 2024 5,400 6 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View