What is the best platform to test the expression of 50 pre selected miRNAs in tumour tissue?

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Korcan Ayata

I would recommend miRNA arrays from Qiagen. Probably, they don't have an array that covers all your miRNAs, in this case you need to order a custom array. It is SYBRG based and uses a common RT method for all miRNA.

Paulo Bettencourt

Dear Evi,

I used Exiqon and TaqMan in the past as well. However, there is a new platform that might be worthwhile to look at, for medium-size arrays, that is Firefly. Please take a look at their products and services.

Kind regards,

Paulo

http://www.fireflybio.com/