I want to make photographs of my plant tissue cultures for publications.
Hello!
You can try to autoclave paper, and wash your bar using alcohol.
Then, you can put your explants onto the autoclave paper in laminar-hood, and put your clean bar nearby.
If you have firther questions don't hesitate to ask me.
The best is to take photos in microscope attached to computer as it has inbuilt software to add scale bars, and also the image clarity is better which is required for tissue culture publications.
But if you don't have that set-up, you can take pictures on any light microscope and simultaneously take pictures of ruler scale at different magnification (which are used for clicking photos).
Then using image J you can add scale bar, it's free software
https://imagej.nih.gov/ij/download.html
All the best
The best shots of tissue culture raised plants come on red background. and size bar can be added by paint or you may keep a scale or ruler.
Each and every stage and all the observations should be documented as photographs. find a good place for photography, it is an important step as it results depend on or are evidenced by the photos.
Adjust light sources to get good photos. Use a ruler while taking photos and finally you can draw scale bars according to the ruler sizes. If it is microscopic images, scale bars are available in the software itself.
Always try to use a black or red background. Put a scale next to the petri dish so that you can use it in Photoshop software for drawing a bar scale.
Kokila Wickramanayake quality photographs are a must to publish your results in a journal. Select a perfect background color and source of light since the cultures are in glass jars or bottles only. If the cultures are of somatic embryos or micro shoots use a stereo microscope for better pictures. For scale bars follow Dr. Manokari's advice, this will help you practically.
You can refer Chapter Digital Photography as a Tool of Research and Documentation ...
https://www.jstor.org/stable/4293788
Photography as a Tool of Research and Documentation in Plant Tissue Culture
An excellent paper, will help you out. Simple methods for improving the results of photography in plant tissue culture are discussed, along with photographic equipment, photomacrography, stereophotomicrography, suitable backgrounds for photography, use of a digital scanner, and the construction of photographic plates.
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