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I have tried to establish shoot cultures of L. reticulata using nodes as an explant, but since December no bud break was observed even in medium in which shoot formation was observed earlier. The...
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Can we add salicylic acid in culture media prior to autoclaving or we should first filter sterilize SA and than it should be added to the media? I have read that the melting point of SA is...
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I am working on Leptadenia reticulata and I have treated the explants with PVP and streptomycin before inoculation. But I have observed off- white growth in agar just below the cut end. I have...
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I have done TLC of my plant extract and done spectral analysis in HPTLC instrument. I have checked spectra of my sample and compared with standard rutin sample which showed 2 peaks one at 254nm...
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I want to elicit my cultures using MJ, which concentrations are being generally used. and how to prepare MJ stock solution? and is it necessary to filter sterilize it before adding to medium?
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