Tring to develop an alternative source to extract bioactive compounds from endangered medicinal plants. Callus cultures will be one of the options. But having a doubt of whether the undifferentiated plant cells so-called callus will be able to produce bioactive compounds as they are found in the differentiated plant tissues?
Yes definitely, secondary metabolites production from cell lines is one of the most useful and promising source. There are many review and articles on same.
But try to take samples from different PGR combinations as it is known to affect the metabolite synthesis. So you need to optimise media for optimum yield. Further you can also develop cell suspension culture and try to enhance the production via elicitor treatment.
All the best.
Yes, Plants can produce both primary and secondary metabolites and the use of plant tissue culture methods for the production of secondary metabolites is very much common. Different plant-based production methods like callus or suspension culturIe is being widely used for the production of secondary metabolites from plants.
These secondary metabolites like alkaloids, phenolics and essential oils play very important role in plant metabolism, i.e. pathogen, insect defense, pigmentation, compounds against herbivores, regulation in germination, growth and development and can also be used by humans for the production of effective drugs and medicine.
In the recent past, various new methods have been cited which tend to have a more important and renounced effect on the production of secondary metabolites.
Besides plant tissue culture, other technologies like Crispr Cas, nano technology and genome editing are also being used for secondary metabolites production.
These plant tissue culture techniques are the best suitable environment friendly techniques that helps in the production of secondary metabolites when chemical synthesis is not feasible or when the natural supply is limited.
Production may occur. However, Concentration may be different from differentiated plants.
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