Sodium alginate is a natural polysaccharide product extracted from brown seaweed that grows in cold water regions. Sodium alginate is a gum, extracted from the cell walls of brown algae. Please have a look at enclosed PDF...
Hi Alberta,
Once the hydrogel is formed, it is essential to maintain the scaffold in buffered medium with calcium. You can use regular cell culture medium or DPBS w/Ca++ and Mg++.
Hope this helps...
PBS is not recommended. You can even store the gel (w/o cells) in water.
For cryopreservation, use both DMSO and Ca2+.
I find normal saline to be a helpful starting point for all solutions. Tonicity will be important to prevent swelling. If you makeup the alginate in normal saline and you make the gelation bath with a divalent cation added to that same normal saline solution you help minimize swing in tonicity. Here is a link to a protocol that may prove useful. Full version on researchgate. https://www.nature.com/articles/nprot.2011.352
You may possibly want to check also the following somewhat related discussions: https://www.researchgate.net/post/Does_change_in_pH_of_sodium_alginate_affect_final_encapsulated_cell_viability https://www.researchgate.net/post/How_can_I_make_alginate_disks_that_I_can_put_into_a_24-well_plate/1
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