Hi !
I am finding the optimum temperature and pH of thermostable enzyme and its mutants. I find the optimum temperature of wild type enzyme and its mutants as 80C and 70C at 10 minutes incubation. but for thermal stability when I incubated the wild type enzyme at three different temperatures as 70,80 and 90 degree centigrade for 4 hours incubation , I find that the enzyme completely lost its activity after 10 minutes incubation for 90C and 80c of 4 hour incubation while for 70c it gives uniform decline in activity with time .
Please let me know why the enzyme is losing its activity at its optimum temperatures ? The enzyme was showing optimum activity at 80C, when I was finding its optimum temperature but when I used this 80C temperature for its thermal stability at four hours incubation without substrate then I find that up to 10 minutes it showing activity but after 10 minutes it completely lost its activity .
can any one tell me the reason or any suggestions for using other methods for accurate optimum temperature and thermal stability ?
thanks
Not my field but it sound like reaction rate and decomposition rate may both increase with increasing temperature. Is it possible that substrate may protect an enzyme from thermal degradation?
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH